Thus Verbascum phoeniceum and nigrum are self-sterile, whilst V. thapsus and lychnitis are quite self-fertile, as I know by trial. There is the same difference between some of the species of Papaver, Corydalis, and of other genera. Nevertheless, the tendency to self-sterility certainly runs to a certain extent in groups, as we see in the genus Passiflora, and with the Vandeae amongst Orchids.
Self-sterility differs much in degree in different plants. In those extraordinary cases in which pollen from the same flower acts on the stigma like a poison, it is almost certain that the plants would never yield a single self-fertilised seed. Other plants, like Corydalis cava, occasionally, though very rarely, produce a few self-fertilised seeds. A large number of species, as may be seen in Table 9/F, are less fertile with their own pollen than with that from another plant; and lastly, some species are perfectly self-fertile. Even with the individuals of the same species, as just remarked, some are utterly self-sterile, others moderately so, and some perfectly self-fertile. The cause, whatever it may be, which renders many plants more or less sterile with their own pollen, that is, when they are self-fertilised, must be different, at least to a certain extent, from that which determines the difference in height, vigour, and fertility of the seedlings raised from self-fertilised and crossed seeds; for we have already seen that the two classes of cases do not by any means run parallel. This want of parallelism would be intelligible, if it could be shown that self-sterility depended solely on the incapacity of the pollen-tubes to penetrate the stigma of the same flower deeply enough to reach the ovules; whilst the greater or less vigorous growth of the seedlings no doubt depends on the nature of the contents of the pollen-grains and ovules. Now it is certain that with some plants the stigmatic secretion does not properly excite the pollen-grains, so that the tubes are not properly developed, if the pollen is taken from the same flower. This is the case according to Fritz Muller with Eschscholtzia, for he found that the pollen-tubes did not penetrate the stigma deeply; and with the Orchidaceous genus Notylia they failed altogether to penetrate it. (9/14. 'Botanische Zeitung' 1868 pages 114, 115.)
With dimorphic and trimorphic species, an illegitimate union between plants of the same form presents the closest analogy with self-fertilisation, whilst a legitimate union closely resembles cross-fertilisation; and here again the lessened fertility or complete sterility of an illegitimate union depends, at least in part, on the incapacity for interaction between the pollen-grains and stigma. Thus with Linum grandiflorum, as I have elsewhere shown, not more than two or three out of hundreds of pollen-grains, either of the long-styled or short-styled form, when placed on the stigma of their own form, emit their tubes, and these do not penetrate deeply; nor does the stigma itself change colour, as occurs when it is legitimately fertilised. (9/15. 'Journal of the Linnean Society Botany' volume 7 1863 pages 73-75.)
On the other hand the difference in innate fertility, as well as in growth between plants raised from crossed and self-fertilised seeds, and the difference in fertility and growth between the legitimate and illegitimate offspring of dimorphic and trimorphic plants, must depend on some incompatibility between the sexual elements contained within the pollen-grains and ovules, as it is through their union that new organisms are developed.
If we now turn to the more immediate cause of self-sterility, we clearly see that in most cases it is determined by the conditions to which the plants have been subjected. Thus Eschscholtzia is completely self-sterile in the hot climate of Brazil, but is perfectly fertile there with the pollen of any other individual. The offspring of Brazilian plants became in England in a single generation partially self-fertile, and still more so in the second generation.